| Date | Home | Away | Kick-off |
|---|---|---|---|
| 5th August, 2017 | Albion Rovers | Ayr United | 3:00 pm |
| 12th August, 2017 | Ayr United | Forfar Athletic | 3:00pm |
| 19th August, 2017 | Stranraer | Ayr United | 3:00pm |
| 26th August, 2017 | Ayr United | Arbroath | 3:00pm |
| 9th September, 2017 | Raith Rovers | Ayr United | 3:00pm |
| 16th September, 2017 | Ayr United | Alloa Athletic | 3:00pm |
| 23rd September, 2017 | Queens Park | Ayr United | 3:00pm |
| 30th September, 2017 | Ayr United | East Fife | 3:00pm |
| 14th October, 2017 | Ayr United | Airdrieonians | 3:00pm |
| 21st October, 2017 | Forfar Athletic | Ayr United | 3:00pm |
| 28th October, 2017 | Ayr United | Stranraer | 3:00pm |
| 4th November, 2017 | Arbroath | Ayr United | 3:00pm |
| 11th November, 2017 | Ayr United | Raith Rovers | 3:00pm |
| 25th November, 2017 | Alloa Athletic | Ayr United | 3:00pm |
| 2nd December, 2017 | Ayr United | Albion Rovers | 3:00pm |
| 9th December, 2017 | Ayr United | Queens Park | 3:00pm |
| 16th December, 2017 | East Fife | Ayr United | 3:00pm |
| 23rd December, 2017 | Airdrie | Ayr United | 3:00pm |
| 30th December, 2017 | Ayr United | Forfar Athletic | 3:00pm |
| 2nd January, 2018 | Stranraer | Ayr United | 3:00pm |
| 6th January, 2018 | Ayr United | Arbroath | 3:00pm |
| 13th January, 2018 | Raith Rovers | Ayr United | 3:00pm |
